Systematic validation of zucker diabetic-sprague dawley male rats as a model for type 2 diabetes-associated diabetic retinopathy. Download PDF Abstract The Zucker Diabetic Sprague-Dawley (ZDSD) rat is an emerging model for type 2 diabetes, but its suitability for diabetic retinopathy (DR) research remains unvalidated. This study aimed to perform a systematic, multimodal characterization of DR in the ZDSD rats to assess its translational relevance.
Male ZDSD rats were fed a high-fat diet to induce diabetes, forming Diabetic and Normoglycemic Control groups ( n = 8 per group), alongside healthy Sprague-Dawley control ( n = 8). Metabolic parameters were monitored from 27 to 36 weeks. Retinal assessment combined in vivo imaging (OCTA, FFA, OCT), histology (H&E staining), and molecular biology (qRT-PCR, Western blot, IHC, ELISA) to quantify key DR mediators: inflammatory factors (TNF-ฮฑ, IL-1ฮฒ, IL-6), VEGF, and Caspase-3.
Diabetic ZDSD rats exhibited severe hyperglycemia, polydipsia, and weight loss. Retinal imaging revealed significant pathology, including progressive reduction in vascular density (OCTA), retinal thinning (OCT), and microvascular leakage (FFA). Histology confirmed structural disorganization and reduced retinal ganglion cell and photoreceptor density.
Medical Disclaimer: This article is for informational purposes only and does not constitute medical advice. Always consult with qualified healthcare professionals for medical decisions and treatment options.
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